Optimization formula and evaluation of polyherbal cream for antimicrobial and wound healing activity

The aim of the research is to develop a preparation formula and evaluate the antibacterial and wound healing capabilities of the product polyherbal cream. The optimal ratio of ingredients polyherbal cream formulation was found by testing the antibacterial properties based on the evaluation of the diameter of the antibacterial zone of different bacterial strains and evaluating the acute toxicity and wound healing ability of polyherbal cream in a mouse model. The research has produced the optimal formulation of polyherbal cream, which includes tamanu oil, tea tree essential oil, and cajeput essential oil at concentrations of 28%, 1%, and 9%, respectively, along with excipients such as hydroxyethyl acrylate (1 g), PEG-600 stearate (3.95 g), and cetyl alcohol (5.45 g) in a 100 g preparation. Polyherbal cream determined the acute skin toxicity dose on mice greater than 2000 mg/kg and evaluated the wound healing ability of the preparation. A formula for the preparation of polyherbal cream has been developed and tested for its antibacterial and wound healing effects, contributing to orienting further research on the beneficial pharmacological effects of products made from tamanu (Calophyllum inophyllum), cajeput (Melaleuca quinquenervia), and tea tree (Melaleuca alternifolia) in Vietnam.