RP-HPLC Method Development and Validation for Simultaneous Estimation of Pravastatin and Bempedoic acid in Bulk and Pharmaceutical Formulation

A simple, rapid, accurate and precise reverse phase high performance liquid chromatographic technique has been progressed for the determination of Pravastatin and Bempedoic acid in absolute form and tablet. Separation of drug peaks were achieved on a Gemini C18 1μ10 Å, LC Ea 150mm x 4.6mm; 5μm column using a mixture of 0.025mM KH2PO4 PH 2.5 and Acetonitrile (60:40) as the mobile phase at rate of flow 1.0ml/min, the recognition was accomplished at 272nm. The retention time of the Pravastatin and Bempedoic caid was 2.398 and 4.801min correspondingly. The approach generates linear reaction in the concentration series of 6-30µg/ml of Pravastatin and 36-270µg/ml of Bempedoic acid. The precision for the assay was below 2.0% RSD. The %recovery for the accuracy was in between 99-101%. The method will be useful in the quality check assessment of Pravastatin and Bempedoic acid in bulk and pharmaceutical formulations.