A comparative analysis of sequence composition in different lots of a phage display peptide library during amplification
Our results highlight tremendous heterogeneity in the peptide composition of different lots of the same type of naïve phage display library, and the divergent evolution of their compositional features during amplification rounds at the amino acid, peptide, and motif levels. Our findings can be instrumental for phage display researchers by bringing fundamental insights into the vast extent of non-uniformity between phage display libraries and by providing a clear picture of how these discrepancies can lead to different evolutionary fates for the peptide composition of phage pools, which can have profound impacts on the outcome of phage display selections through biopanning.
Authors
- Amanda Oester Andersen (ORCID: https://orcid.org/0000-0002-4676-0369)
- Anders Wilgaard Sinkjær (ORCID: https://orcid.org/0000-0001-6287-481X)
- Andreas Kjær (ORCID: https://orcid.org/0000-0002-2706-5547)
- Babak Bakhshinejad (ORCID: https://orcid.org/0000-0002-5024-6688)
- Ane Beth Sloth (ORCID: https://orcid.org/0000-0001-6891-9812)
- Malte Jensen (ORCID: https://orcid.org/0000-0003-0555-4216)
Institutions
- University of Copenhagen (DK)
- Copenhagen University Hospital (DK)
- Rigshospitalet (DK)
Publication Details
- Journal
- Virology Journal
- Published
- 2025-02-01
- DOI
- https://doi.org/10.1186/s12985-024-02600-x
- Citations
- 4
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 5.44
Funders
- Arvid Nilssons Fond
- Kræftens Bekæmpelse
- Neuroendocrine Tumor Research Foundation
- Innovationsfonden
- Danmarks Grundforskningsfond
- Københavns Universitet
- Lundbeckfonden
- Novo Nordisk Fonden