Two antagonistic gene regulatory networks drive Arabidopsis root hair growth at low temperature linked to a low‐nutrient environment
Root hair (RH) cells can elongate to several hundred times their initial size, and are an ideal model system for investigating cell size control. Their development is influenced by both endogenous and external signals, which are combined to form an integrative response. Surprisingly, a low-temperature condition of 10°C causes increased RH growth in Arabidopsis and in several monocots, even when the development of the rest of the plant is halted. Previously, we demonstrated a strong correlation between RH growth response and a significant decrease in nutrient availability in the growth medium under low-temperature conditions. However, the molecular basis responsible for receiving and transmitting signals related to the availability of nutrients in the soil, and their relation to plant development, remain largely unknown. We have discovered two antagonic gene regulatory networks (GRNs) controlling RH early transcriptome responses to low temperature. One GNR enhances RH growth and it is commanded by the transcription factors (TFs) ROOT HAIR DEFECTIVE 6 (RHD6), HAIR DEFECTIVE 6-LIKE 2 and 4 (RSL2-RSL4) and a member of the homeodomain leucine zipper (HD-Zip I) group I 16 (AtHB16). On the other hand, a second GRN was identified as a negative regulator of RH growth at low temperature and it is composed by the trihelix TF GT2-LIKE1 (GTL1) and the associated DF1, a previously unidentified MYB-like TF (AT2G01060) and several members of HD-Zip I group (AtHB3, AtHB13, AtHB20, AtHB23). Functional analysis of both GRNs highlights a complex regulation of RH growth response to low temperature, and more importantly, these discoveries enhance our comprehension of how plants synchronize RH growth in response to variations in temperature at the cellular level.
Authors
- José M. Estevez (ORCID: https://orcid.org/0000-0001-6332-7738)
- Ayako Kawamura (ORCID: https://orcid.org/0000-0001-9218-7850)
- Gerardo Núñez‐Lillo (ORCID: https://orcid.org/0000-0001-5309-1565)
- Keiko Sugimoto (ORCID: https://orcid.org/0000-0002-9209-8230)
- Miguel Angel Ibeas (ORCID: https://orcid.org/0000-0003-0274-0662)
- Adrián A. Moreno (ORCID: https://orcid.org/0000-0001-5125-2927)
- Hernán Salinas‐Grenet
- Tomás C. Moyano (ORCID: https://orcid.org/0000-0001-6495-4995)
- Miguel Angel Rosas (ORCID: https://orcid.org/0000-0002-9686-7391)
- Karen Sanguinet (ORCID: https://orcid.org/0000-0002-1226-1422)
- Trevor M. Nolan (ORCID: https://orcid.org/0000-0003-1362-2557)
- Lucía Ferrero (ORCID: https://orcid.org/0000-0002-1558-6476)
- Fiorella Paola Spies (ORCID: https://orcid.org/0009-0001-3410-0339)
- Tomás Urzúa Lehuedé (ORCID: https://orcid.org/0009-0009-5119-2843)
- María Florencia Perotti (ORCID: https://orcid.org/0000-0002-2523-3965)
- Diana Rosa Rodríguez Garcia
- Victoria Berdion Gabarain (ORCID: https://orcid.org/0000-0003-4911-6090)
- Claudio Meneses (ORCID: https://orcid.org/0000-0002-6452-8950)
- Raquel L. Chan (ORCID: https://orcid.org/0000-0002-3264-0008)
- Norbert Perrimon (ORCID: https://orcid.org/0000-0001-7542-472X)
- Federico Ariel (ORCID: https://orcid.org/0000-0001-8478-8808)
- Virginia Natalí Miguel (ORCID: https://orcid.org/0009-0008-9546-6677)
- José M. Álvarez (ORCID: https://orcid.org/0000-0002-5073-7751)
- Ah‐Ram Kim (ORCID: https://orcid.org/0000-0001-9597-6759)
- Jorge Pérez‐Díaz
- Romina Achá‐Escobar
Institutions
- California Institute of Technology (US)
- Pontificia Universidad Católica de Valparaíso (CL)
- Howard Hughes Medical Institute (US)
- Harvard University (US)
- Universidad Andrés Bello (CL)
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Pontificia Universidad Católica de Chile (CL)
- Fundación Instituto Leloir (AR)
- Millennium Science Initiative (CL)
- Universidad Nacional del Litoral (AR)
- Health First (US)
- RIKEN Center for Sustainable Resource Science (JP)
- Boston VA Research Institute (US)
- Instituto de Agrobiotecnología del Litoral (AR)
- Millennium Institute for Integrative Biology (CL)
- Washington State University (US)
- The University of Tokyo (JP)
Publication Details
- Journal
- New Phytologist
- Published
- 2025-02-01
- DOI
- https://doi.org/10.1111/nph.20406
- Citations
- 6
- Primary Topic
- Plant Molecular Biology Research
- Type
- article
- Field-Weighted Citation Impact
- 10.50
Funders
- Fondo para la Investigación Científica y Tecnológica
- Fondo Nacional de Desarrollo Científico y Tecnológico