Antioxidants Activity of Face Toner from Ethyl Acetate Fraction of Okra Seeds

This study explores the potential of okra seeds (Abelmoschus esculentus L.) as a natural face toner, using maceration extraction method.A total of 750 g of Okra was dried for 72 h using methanol solvent showed that okra seeds contain flavonoids with 5% FeCl reagent.FTIR analysis revealed a characteristic functional group profile of flavonoids, including hydroxyl (3400 cm-1), carbonyl (1722 cm-1), and aromatic ring (1591 cm-1 and 1277 cm-1) groups.The antioxidant test was selected from the ethyl acetate fraction at a wavelength of 517 nm with an IC50 value of 44.90 g/mL, and can be categorized as a very strong antioxidant.DPPH test of toner formula with BHT, ethyl acetate extract, and control produced IC50 values of 85.67 g/mL, 88.79 g/mL, 105.69 g/mL, respectively.The optimal conditions were achieved with 25, 50, and 100 ppm of ethyl acetate fractions.Future studies could expand on these findings by exploring the long-term effects and consumer acceptability of okra seed toners in broader populations.