Chemical-state imaging of a mammalian cell through multi-elemental soft x-ray spectro-ptychography
Soft x-ray spectro-ptychography is a high-resolution chemical state imaging technique and has significant potential for the analysis of light-element-rich samples such as biological cells. We measured hyperspectral images of a whole mammalian neuronal cell at the nitrogen and oxygen absorption edges with soft x rays using the achromatic ptychography system CARROT developed at SPring-8 BL07LSU. We visualized and classified the intracellular structures based on the difference of chemical states, which are difficult to recognize in a monochromatic image. The method is expected to give insight into the relationship between intracellular structures and chemical states.
Authors
- Takenori Shimamura (ORCID: https://orcid.org/0000-0002-4144-2206)
- Noboru Furuya
- Satoru Egawa (ORCID: https://orcid.org/0000-0003-0509-597X)
- Haruhiko Ohashi (ORCID: https://orcid.org/0000-0003-4904-9107)
- Yoshihisa Harada (ORCID: https://orcid.org/0000-0002-4590-9109)
- Yoko Takeo (ORCID: https://orcid.org/0000-0003-4688-326X)
- Kyota Yoshinaga
- Kazuyoshi Yoshimi (ORCID: https://orcid.org/0000-0002-6249-6844)
- Kai Sakurai (ORCID: https://orcid.org/0000-0002-7418-6333)
- Mari Shimura
- Hidekazu Mimura (ORCID: https://orcid.org/0000-0003-4623-6951)
- Keiichi Inoue (ORCID: https://orcid.org/0000-0002-6898-4347)
- Shunki Takaramoto (ORCID: https://orcid.org/0000-0002-8195-3115)
- Takashi Kimura (ORCID: https://orcid.org/0000-0001-7890-7719)
- Jordan T. O’Neal
- Yu Nakata
Institutions
- National Center for Global Health and Medicine (JP)
- SPring-8 (JP)
- Japan Synchrotron Radiation Research Institute (JP)
- The University of Tokyo (JP)
Publication Details
- Journal
- Applied Physics Letters
- Published
- 2025-01-27
- DOI
- https://doi.org/10.1063/5.0237804
- Citations
- 1
- Primary Topic
- Advanced X-ray Imaging Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.88
Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency
- Core Research for Evolutional Science and Technology
- Precursory Research for Embryonic Science and Technology