Molecular characterization of pea (Pisum sativum) genotypes by utilization of RAPD markers

A Random Ampli ied Polymorphic DNA (RAPD) marker was used for identifying and mapping the population in pea (Pisum sativum).The presence of multiple polymorphisms between cultivars and lines revealed at least one fragment for any given primer was present in the DNA of one form of pea and absent in the DNA of another line or cultivar.Polymerase chain reaction (PCR) based molecular marker viz.random ampli ied polymorphic DNA was applied to 20 germplasm of Pea to assess the degree of polymorphism within the genes and to investigate the genetic studies in Pea.This study, using 20 germplasm of pea was evaluated for variability using a panel of 14 random l0-mer primers.The polymorphisms in PCR ampli ication products were subjected to the unweighted pair group method for arithmetic averages (UPGMA) and plotted in a dendrogram based on similarity data showing that all the cultivars analyzed were related.Eleven out of 14 primers revealed scorable 60-polymorphic bands between cultivars of Pisum sativum and the rest did not show polymorphism in their genetic level.All the 60 ampli ied bands were polymorphic and the numbers of bands produced per primer ranged from band 3 to 11 bands.PIC, EMR, and MI values ranged from 0.22 to 0.37, 1.00 to 5.20, and 0.34 to 1.92 with the average of PIC, EMR, and MI values being 0.34, 2.86, and 0.95 respectively.In addition, the value of resolving power (RP) ranged from 0.80 to 6.20 with an average value of 2.59.GS (Genetic similarity) value ranged from 0.13 between genotypes VL-3 and Arka Ajit and 0.90 between genotype AP-3 and Arka Priya.