Antimicrobial Assessment of Biopolymer Based Scaffold Prepared by Freeze-Thaw Process for Various Biomedical and Pharmaceutical Applications

This present research work is based on soya protein isolates biopolymer cryogel, which was synthesized by the freeze thaw method. Cryogel is a porous three-dimensional network material that is insoluble in water and can store much water. These materials have excellent mechanical properties, such as in medical fields, wastewater treatment, and other fields, and are widely used. With the development of modern science and technology, the research on hydrogel, a new kind of biomaterial, is increasingly extensive. Many researchers have researched bone tissue engineering based on the advantages of hydrogel, such as its adjustable mechanical properties, convenient production, and combination with bioactive factors. This method forms a spongy, flexible, and macroporous gel characterized by the Scanning Electron Microscopy technique (SEM). The morphology of the cryogel surface depicted irregular pores structure. After drug loading, the surface of the gel is relatively smoother with no voids and interconnected pores, showing the uniform distribution of and good adsorption of the antibiotic drug into the gel. Surface roughness parameters investigated by Atomic Force Microscopy (AFM). The particle size of gel and drug particles dispersed into gel were also studied using Transmission Electron Microscopy (TEM). Drug loaded gel examined by antimicrobial spectrum against gram positive and gram negative bacteria. The prepared drug loaded gel is used in wound dressing, target drug delivery, food packaging and tissue engineering.