Development of a UV Spectrophotometric Method for Validating Drug Loading Efficiencyof SNEDDS Rifampicin

Efficient and cost-effective UV spectrophotometric techniques have been devised using methanol and phosphate buffer pH 7.4 to quantify Rifampicin concentrations in the Self Nanoemulsifying Drug Delivery System (SNEDDS), as shown by the drug loading efficiency. Rifampicin has a peak wavelength of 474nm with a concentration of 10-50µg/mL. The relationship between the concentration and the absorbance is linear, as shown by a high correlation value (r) of 0.9998. The average percent recovery of Rifampicin falls between the range of 99.138% to 101.349%. The approach devised is suitable for analyzing Rifampicin in nanoparticle formulations.