GENERATION OF REACTIVE OXYGEN SPECIES BY LEUKOCYTES IN ABNORMAL UTERINE BLEEDING DURING PUBERTY

Abnormal uterine bleeding during puberty is a complex impairment affecting life quality and requiring an in-depth study of pathogenesis. AUB accounts for 50% of all gynecological diseases in the pubertal period. Purpose. To investigate the peculiarities of the intensity of generation of reactive oxygen species in leukocytes of peripheral blood during abnormal uterine bleeding in the pubertal period. Materials and methods of the study. The study involved 31 patients aged 10 to 17 years with AUB, who made up the main group (MG), and 12 somatically healthy girls (control group (CG)). Depending on the level of hemoglobin in the complete blood count, MG patients were further divided into three subgroups. The degree of anemia severity was determined by hemoglobin level in the clinical blood test and presentation of the patients (weakness, headache, dizziness, pale skin, decreased performance at school). With the help of flow cytometry, the level of ROS generation in leukocytes was assessed, the redox potential of the studied cells was determined,. Results. As a result of the conducted study, it was determined that in patients with AUBPP and grade I anemia, the generation of ROS in leukocytes did not differ from DCF MFI indicator in CG subjects. The generation of ROS in the blood leukocytes of patients with AUBPP and with grade II anemia significantly exceeded this indicator in CG and in patients with grade I anemia by 232.3% and 254.1%, respectively. The highest level of ROS generation in leukocytes was observed in patients with AUBPP and grade III anemia. The study showed an increase in the content of ROS in leukocytes by 13.5 and 14.4 times compared to CG and group 1, respectively. When compared with the DCF MFI indicator in leukocytes of Group 2 patients, the generation of ROS was 4.1 times higher. Conclusions. The highest level of ROS generation in leukocytes occurs in patients with AUBPP and II and III grade anemia. Increased generation of ROS in peripheral blood leukocytes contributes to cell death, which can subsequently lead to a decrease in the body's immune status and the development of acute and chronic pathological processes in the body.