Application of OUT (Onco-Ultrasound-Tripsy) Procedure to Cancer cells: Results on Lymphoma and Melanoma Cells

In recent years, significant progress has been made in cancer research, leading to improved treatments for various tumour types and a longer life expectancy for patients. Despite this, there remains strong interest in exploring alternative clinical approaches. A key challenge is developing treatments that specifically target tumour without affecting surrounding healthy tissue. Numerous studies have demonstrated that low-intensity ultrasound can selectively damage tumour cells due to their distinct mechanical properties if compared to healthy counterpart. This opens the way to a procedure referred as onco-ultrasound-tripsy consisting in exposing cells to ultrasound vibration to induce selective mechanical damage. In this study, we evaluated the effects of ultrasound on two different types of cells: U937 Human Histiocytic Lymphoma cells, while preserving the viability of healthy Human CD14+ Monocytes as a control. Various sonication settings were tested to determine the optimal conditions for selectively killing U937 cancer cells. Similar approach was also adopted for preliminary investigations of onco-ultrasound-tripsy effects on HBL melanoma cells.