P-1368. High prevalence of class 1 integrons in carbapenem resistant <i>Enterobacterales</i> in Taiwan

Abstract Background The prevalence of integron in carbapenem resistant Enterobacterales is not well studied. This study aimed to investigate the prevalence and resistance gene cassettes of class 1 integron in carbapenem resistant Enterobacterales.Fig. 1.Integron gene expression in Carbapenem-resistant Enterobacterales (CRE)(N=101). Methods In total, 101 carbapenem resistant Enterobacterales strains were collected through 2020 to2023. Genomic DNA was extractedand PCR method was performed for detection of class 1 integron gene cassettes. Sequences were performed by an automatic sequencer with Applied Biosystems 3730xl and alignment in GenBank.Figure 2.Resistance gene cassettes of Integron(+) in carbapemase-producing and non-carbapenemase producing carbapenem-resistant Klebsiella pneumoniae Results The prevalence of class 1 integron cassette of all Carbapenem resistant Enterobacterales (CRE) was 79.21% (80/101), and was high as 83% (44/53) in carbapenem resistant Klebsiella pneumoniae.The most common resistant gene cassettes wer edfrA12-aadA2 (20 %), following by dfrA17-aadA5 (17.5%) and aadA2 (11.25%). No carbapenemase gene was detected in class 1 integron gene cassettes. However, acriflavine resistance B (AcrB) gene, which is a multiple efflux transporter, wasdetected in 4 strains.Table 1.The expression patterns of integron genes (+) and integron genes (-) cassettes in different Carbapenem-resistant Enterobacterales (CRE). Conclusion Our study revealed high prevalence of type I integron gene cassettes in carbapenemase resistance Enterobacterales. The role of AcrB gene need to be further studied. Disclosures All Authors: No reported disclosures