A Pilot Study on Qualitative Metabolomics to Characterize Lewis Lung Carcinoma in Mice
Metabolomics is a powerful tool that can be used to identify different stages in cancer development. In this study, the metabolomic profile of Lewis lung carcinoma (LLC) was characterized in C57BL/6 mice bearing LLC tumors. Magnetic resonance spectroscopy (nuclear magnetic resonance-NMR) was applied using a 400 MHz <sup>1</sup>H NMR spectrometer. Two types of metabolites (polar and non-polar) were identified on LLC based on the analysis of methanol/water and chloroform extracts collected from lung cancer samples in mice. The investigated metabolomics show that the neoplastic processes of growing LLC on mice may affect carbohydrate; alanine and glutamate; leucine and isoleucine; lysine; creatine; and choline metabolism, whereas hypoxia states were identified due to elevated lactate in lung cancer tissues. The metabolomic profile of Lewis lung carcinoma could be considered to be a valuable biomarker in translational lung cancer research.
Authors
- Maciej Gawlak (ORCID: https://orcid.org/0000-0002-7241-6326)
- Dariusz Maciej Pisklak (ORCID: https://orcid.org/0000-0002-4648-9513)
- Magdalena Bamburowicz‐Klimkowska (ORCID: https://orcid.org/0000-0003-2213-415X)
- Agnieszka Stawarska (ORCID: https://orcid.org/0000-0001-9345-1254)
- Ireneusz P. Grudziński (ORCID: https://orcid.org/0000-0002-6113-1771)
Institutions
- Medical University of Warsaw (PL)
Publication Details
- Journal
- Life
- Published
- 2025-01-29
- DOI
- https://doi.org/10.3390/life15020202
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00