Imaging Single Particle Profiler to Study Nanoscale Bioparticles Using Conventional Confocal Microscopy
Single particle profiling (SPP) is a unique methodology to study nanoscale bioparticles such as liposomes, lipid nanoparticles, extracellular vesicles, and lipoproteins in a single particle and high throughput manner. The initial version requires the single photon counting modules for data acquisition, which limits its adoptability. Here, we present imaging-based SPP (iSPP) that can be performed by imaging a spot over time in the common imaging mode with confocal detectors. We also provide user-friendly software with a graphical user interface to analyze such data and give quantitative insights on the content and properties of nanoscale bioparticles. We use iSPP to decipher lipid-protein interactions, membrane modifications by drugs, and the heterogeneity of extracellular vesicles isolated from cell lines and human urine. This easily applicable modality of the single particle profiler will facilitate nanoscale bioparticle research in laboratories with access to any confocal microscope.
Authors
- Erdinç Sezgin (ORCID: https://orcid.org/0000-0002-4915-388X)
- André Görgens (ORCID: https://orcid.org/0000-0001-9198-0857)
- Gözde Güçlüler
- Markus Johansson
- Amir M. Sherif (ORCID: https://orcid.org/0000-0002-3675-3050)
- Ylva Huge (ORCID: https://orcid.org/0009-0004-2332-0653)
- Loïc Steiner (ORCID: https://orcid.org/0000-0001-6640-3513)
- Susanne Gabrielsson (ORCID: https://orcid.org/0000-0003-1771-1346)
- Samir EL Andaloussi (ORCID: https://orcid.org/0000-0003-4468-9113)
- Firas Aljabery (ORCID: https://orcid.org/0000-0002-8072-3625)
- Taras Sych (ORCID: https://orcid.org/0000-0002-7330-9063)
- Farhood Iranparvar Alamdari (ORCID: https://orcid.org/0000-0003-3589-7789)
Institutions
- Linköping University (SE)
- Karolinska University Hospital (SE)
- Science for Life Laboratory (SE)
- Karolinska Institutet (SE)
- Institute for Transfusion Medicine (US)
- Västmanlands sjukhus Västerås (SE)
- Länssjukhuset Sundsvall-Härnösand (SE)
- County Administrative Board (SE)
- Early Intervention Foundation (GB)
- Respiratory Clinical Trials (AU)
- Pediatrics and Genetics (US)
- Umeå University (SE)
Publication Details
- Journal
- Nano Letters
- Published
- 2025-01-29
- DOI
- https://doi.org/10.1021/acs.nanolett.4c05117
- Citations
- 6
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 4.84
Funders
- Wellcome Trust
- Swedish Cancer Foundation
- International Society for Advancement of Cytometry
- European Commission
- Human Frontier Science Program
- Karolinska Institutet
- Vetenskapsrådet
- Radiumhemmets Forskningsfonder
- Science for Life Laboratory
- Medical Research Council