mSphere of Influence: High-throughput screens to rapidly assign function to microbial genes

Lori Huberman works in the field of fungal genetics, with an emphasis on investigating the genetic mechanisms fungi use to sense and respond to the nutrients and toxins in their environment. In this mSphere of Influence article, she reflects on how "Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons" by K. M. Wetmore, M. N. Price, R. J. Waters, J. S. Lamson, et al. (mBio 6:e00306-15, 2015, https://doi.org/10.1128/mBio.00306-15) made an impact on her by establishing technologies that open realistic possibilities for developing high-throughput screening methods to correlate phenotype to genotype in diverse fungal species.