Integrated Analysis of Indoor Air Quality and Fungal Microbiota in Educational Heritage Buildings: Implications for Health and Sustainability

Indoor air quality is paramount for the health and well-being of individuals, especially in enclosed spaces like office buildings, schools, hospitals, and homes where people spend a significant amount of time. Ensuring good indoor air quality is not only essential for reducing symptoms such as headaches, eye and respiratory irritation, fatigue, and difficulty in concentration, but it is also a key component of sustainable building practices aimed at promoting long-term health and environmental balance. This study aims to explore the impact of the microclimate and fungal microbiota on the health and cognitive performance of occupants in a university classroom, which is part of a cultural heritage building. The research delves into various microclimatic parameters, including temperature, relative humidity, CO2, volatile organic compounds, O2, and particulate matters (PM2.5 and PM10), to understand their influence on the development of microbiota and the manifestation of symptoms associated with Sick Building Syndrome. Over the course of a year-long investigation, microbiological samples were collected, revealing the presence of 19 fungal species, with Cladosporium, Alternaria, and Aureobasidium being the most prevalent genera. These species were found to thrive in an environment characterized by inadequate ventilation, posing potential health risks to occupants, such as allergic reactions and respiratory infections. Microclimatic parameter values such as mean temperature of 22.9 °C and mean relative humidity of 38.5% indicated moderate conditions for fungal proliferation, but occasional high levels of PM2.5 and CO2 indicated periods of poor indoor air quality, negatively influencing the comfort and health of the occupants. The questionnaires completed by 190 students showed that 51.5% reported headaches, 44.2% frequent sneezing, and 43.7% severe fatigue, linking these symptoms to increased levels of CO2 and PM2.5. The novelty of the study lies in the integrated approach to indoor air quality assessment in a heritage educational building, highlighting the need for improved ventilation and air management to enhance health and cognitive performance, while emphasizing sustainable indoor environment management that balances occupant well-being with the preservation of cultural heritage.