P-1386. Developing a Diagnostic Strategy for the Diagnosis of Bacterial Vaginosis: A Shift from Subjective to Objective Approach

Abstract Background Bacterial vaginosis (BV), a polymicrobial syndrome of unclear etiology is a major public health problem. The current diagnostic approaches lack standardisation and are not easily reproducible. We aim to develop a diagnostic modality to enhance the accurate diagnosis of BV. Methods 385 vaginal samples were categorised into BV positive (n=108), BV negative (n=208) and intermediate group (n=69). The positive and negative samples were then subjected to qualitative and quantitative PCR. The two pathogens targetted were Fannyhessea vaginae and Gardenerella vaginalis. Standardisation real time PCR: CP value and Tm value for Gardnerella vaginalis Results PCR detection as compared to Nugent criteria yielded a sensitivity of 96% and specificity of 82.9%, positive predicitive value (PPV) of 68.5% and negative predicitive value (NPV) of 98.2%. In contrast, molecular quantification of the two bacteria together had highest specificity (99.52%) with excellent PPV of 98.1%. Although, with a low sensitivity (48.1%) and NPV (78%). Conclusion All women in reproductive age group should be screened for BV using qualitative PCR targeting Fannyhessea vaginae and Gardenerella vaginalis together. In symptomatic women, the use of quantitative PCR can provide a consistent diagnosis of BV. Thus this objective diagnostic strategy can play a pivotal role in prevention, early diagnosis, treatment initiation and cure of BV. Disclosures All Authors: No reported disclosures