Rapid, sensitive, and visible RPA-LFD assay for BoHV-1 and BoHV-5
Bovine herpesvirus (BoHV), a significant respiratory pathogen in cattle, is widely prevalent worldwide, posing a substantial economic threat to the cattle industry. In this study, we have developed a rapid on-site detection method for BoHV, termed RPA-LFD, which can carry out pathogen detection with a sensitivity of no less than 1 TCID<sub>50</sub> within 30 min. When combined with screening, isolation, and culling measures, this approach can effectively eliminate the sources of BoHV infection, providing a scientifically feasible solution for BoHV prevention and control.
Authors
- Anding Zhang (ORCID: https://orcid.org/0000-0002-0037-5916)
- Guishan Ye
- Chenyang Yi (ORCID: https://orcid.org/0000-0002-4460-5701)
- Wenyi Zhang (ORCID: https://orcid.org/0000-0002-1107-0587)
- Yue Wu (ORCID: https://orcid.org/0000-0001-8277-0970)
- K. K. He (ORCID: https://orcid.org/0009-0003-4086-716X)
- Changmin Hu
Institutions
- Huazhong Agricultural University (CN)
- Ministry of Science and Technology of the People's Republic of China (CN)
- Center of Hubei Cooperative Innovation for Emissions Trading System (CN)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-01-27
- DOI
- https://doi.org/10.1128/spectrum.00895-24
- Citations
- 1
- Primary Topic
- Herpesvirus Infections and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 1.35
Funders
- National Key Research and Development Program of China