Duplexed direct RNA sequencing protocol using polyadenylation and polyuridylation
Oxford Nanopore Technologies provides multiplexing options for DNA and cDNA sequencing, but not for direct RNA sequencing. Here we describe a duplexing approach and validate it by simultaneously sequencing the <i>Saccharomyces cerevisiae</i> rRNA from wild type and knockout that have differential rRNA modifications, successfully demultiplexing the data using bioinformatics approaches.
Authors
- Kaylee J. Watson (ORCID: https://orcid.org/0009-0008-2288-5723)
- Julie C. Dunning Hotopp (ORCID: https://orcid.org/0000-0003-3862-986X)
- Robin E. Bromley (ORCID: https://orcid.org/0000-0003-2738-2603)
Institutions
- University of Maryland, Baltimore (US)
- U-M Rogel Cancer Center (US)
Publication Details
- Journal
- Microbiology Resource Announcements
- Published
- 2025-01-27
- DOI
- https://doi.org/10.1128/mra.01041-24
- Citations
- 5
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 3.22
Funders
- National Science Foundation
- U.S. Department of Health and Human Services
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases