A validated single-step saliva and serum sample extraction LC-MS/MS method for the analysis of nicotine, cotinine and 3’-hydroxycotinine for clinical vaping studies
The integration of a delay column into the LC-MS/MS method mitigated the interference from environmental nicotine and facilitated the quantification of very low nicotine concentrations and two of its major metabolites in saliva and serum. C18 chromatography and single-step sample extraction make the method stable and suitable for large sample loads.
Authors
- Urs Duthaler
- M. A. Hirt
- Samuel E Christen (ORCID: https://orcid.org/0009-0006-3103-781X)
- Vera van der Velpen (ORCID: https://orcid.org/0000-0002-6502-7197)
- Manuel Haschke (ORCID: https://orcid.org/0000-0003-1846-8640)
- Evangelia Liakoni (ORCID: https://orcid.org/0000-0002-2239-1378)
- Celina Vonwyl
- Peyton Jacob
Institutions
- University of Bern (CH)
- University of California, San Francisco (US)
- University of Basel (CH)
- University Hospital of Bern (CH)
- Department of Biomedicine Basel (CH)
Publication Details
- Journal
- Journal of Pharmaceutical and Biomedical Analysis
- Published
- 2025-01-27
- DOI
- https://doi.org/10.1016/j.jpba.2025.116703
- Citations
- 4
- Primary Topic
- Hormonal and reproductive studies
- Type
- article
- Field-Weighted Citation Impact
- 5.18