Synthesis of Porous Au-Shell-Coated Silica Nanoparticles (SiO<sub>2</sub>@Au@AuPS) under Mild Conditions for Photothermal Therapy and Chemotherapy of Cancer Cells
Multifunctional nanoparticles (NPs) have gained considerable attention for cancer imaging and treatment. Herein, we develop novel multifunctional nanoprobes called SiO2@Au@Au porous shells (SiO2@Au@AuPS). SiO2@Au is fabricated by introducing 4 nm Au NPs to the thiol-modified SiO2 surface, after which alloy shells with different Ag/Au ratios are added. Shell modification is then implemented to obtain a porous structure via Ag etching using a dealloying reaction. Among the NPs, SiO2@Au@AuPS with an Au/Ag ratio of 1.5 (SiO2@[email protected]) has a well-structured porous Au shell, with a number of nanogaps confirmed by the strongest Raman signal. Furthermore, SiO2@Au@AuPS decorated with PEG, 4-MBA, and anti-CD44 antibodies shows photothermal conversion effects under 980 nm, with a maximum drug loading capacity of 75.7 μg mg–1 for doxorubicin (DOX) and a releasing efficiency reaching 53.9% in a pH 6.0 buffer solution within 4 h. In vitro treatment of MDA-MB-231 breast cancer cells with SiO2@[email protected]@PEG/4-MBA/anti-CD44/DOX decreases the cell viability to 20.3% under near-infrared laser irradiation with successful NP delivery and DOX release. Additionally, in vivo photothermal therapy/chemotherapy with tail-vein-injected SiO2@[email protected]@PEG/4-MBA/anti-CD44/DOX is effective for cancer cell treatment. Our developed porous nanostructure presents possibilities for synergetic methods of effective photothermal treatment and drug delivery.
Authors
- Dae Hong Jeong (ORCID: https://orcid.org/0000-0001-7869-5648)
- Jong Sam Lee (ORCID: https://orcid.org/0000-0001-6266-3983)
- Won‐Yeop Rho (ORCID: https://orcid.org/0000-0002-1583-154X)
- Wooyeon Kim (ORCID: https://orcid.org/0000-0001-7077-0412)
- Xuan‐Hung Pham (ORCID: https://orcid.org/0000-0002-4927-7102)
- Jaehi Kim (ORCID: https://orcid.org/0000-0002-5150-4810)
- Seung Min Park (ORCID: https://orcid.org/0000-0002-2123-6245)
- Kyeong-Min Ham
- Hyung‐Mo Kim (ORCID: https://orcid.org/0000-0001-8507-089X)
- Bong‐Hyun Jun (ORCID: https://orcid.org/0000-0001-6556-7946)
- Hyejin Chang (ORCID: https://orcid.org/0000-0003-0569-4155)
- Homan Kang (ORCID: https://orcid.org/0000-0002-0732-9779)
- Sang Hun Lee (ORCID: https://orcid.org/0000-0002-8629-8842)
- Dong‐Eun Kim (ORCID: https://orcid.org/0000-0001-6545-8387)
- Kwanghee Yoo
- Cho-Hee Yang
- Minsup Shin
- Minhee Kim (ORCID: https://orcid.org/0000-0001-9117-3414)
- Yun-Sik Choi
Institutions
- Harvard University (US)
- Seoul National University (KR)
- Hanbat National University (KR)
- Seoul National University of Education (KR)
- Kangwon National University (KR)
- Konkuk University (KR)
- Gordon Center for Medical Imaging (US)
- Massachusetts General Hospital (US)
- Konkuk University Medical Center (KR)
- Advanced Institute of Convergence Technology (KR)
- Stanford Medicine (US)
- Jeonbuk National University (KR)
- Stanford University (US)
Publication Details
- Journal
- ACS Applied Nano Materials
- Published
- 2025-01-27
- DOI
- https://doi.org/10.1021/acsanm.4c06900
- Citations
- 2
- Primary Topic
- Nanoplatforms for cancer theranostics
- Type
- article
- Field-Weighted Citation Impact
- 1.35
Funders
- Ministry of Trade, Industry and Energy
- Ministry of Science and ICT, South Korea