Nutraceutical Impact of Pumpkin Seed Oil on Expression Levels of EZH-2 and KRT-14 Genes against DSS-induced Inflammatory Bowel Disease in the Rat Model
Inflammatory bowel disease is a collection of intestinal disorders that cause inflammation in the digestive tract. Prolonged inflammation in the gastrointestinal tract is a major risk factor for colorectal cancer. The objective of this study was to fucus on gene expression levels of (KRT-14; associated with epithelial cell integrity) and enhancer of zeste homolog-1 (EZH-2; involved in cellular proliferation) in a IBD rat model in order to rule out impact of nutraceuticals (pumpkin seed oil; PSO) as a complementary approach to conventional treatments of IBD. In the current study, IBD was induced using dextran sodium sulfate (DSS). Following acclimatization, rats were separated into three groups: the negative control, the positive control, and the treatment group. The DSS (1 ml/kg bw) was given to the positive control and treatment groups. Negative control was given only a normal diet. Pumpkin seed oil (PSO) was given orally as a treatment (0.5 ml/kg bw). Blood and colon tissue were obtained on the 5<sup>th</sup>, 10<sup>th</sup>, 14<sup>th,</sup> and 18<sup>th</sup> days. Physical parameters, hematology, biochemical assays, gene expression, and histopathology were carried out. After statistical analyses, macroscopic parameters showed significant differences. Biochemical analyses revealed a significant (P ≤ 0.05) decrease in serum potassium concentrations, total cholesterol, triglycerides, total proteins, total oxidants status, and C-reactive proteins in PSO treated group as compared with positive control. Gene expression levels of KRT-14 and EZH2 were significantly (P ≤ 0.05) upregulated in PSO treated group as compared to positive control group. Histopathology revealed that pumpkin seed oil preserved the structural integrity of colon.
Authors
- Laaraib Nawaz
- Humaira Muzaffar (ORCID: https://orcid.org/0000-0003-2172-8474)
- Muhammad Umar Ijaz (ORCID: https://orcid.org/0000-0002-8398-5364)
- Sameh A. Korma (ORCID: https://orcid.org/0000-0001-8385-2599)
- Muhammad Naeem Faisal (ORCID: https://orcid.org/0000-0001-6448-9325)
- Muhammad Umair (ORCID: https://orcid.org/0000-0002-2035-686X)
- Farid Shokry Ataya (ORCID: https://orcid.org/0000-0002-0981-7805)
- Marios Papadakis (ORCID: https://orcid.org/0000-0002-9020-874X)
- Nermeen N. Welson (ORCID: https://orcid.org/0000-0001-7854-2086)
- Sana Maryam
- Αθανάσιος Αλεξίου (ORCID: https://orcid.org/0000-0002-2206-7236)
- Imran Mukhtar
- Kashif-ur-Rehman Khan (ORCID: https://orcid.org/0000-0002-3581-5600)
- Haseeb Anwar
- Asma Mukhtar
- Gaber El-Saber Batiha
Institutions
- Chandigarh University (IN)
- Beni-Suef University (EG)
- Witten/Herdecke University (DE)
- Damanhour University (EG)
- Islamia University of Bahawalpur (PK)
- Zagazig University (EG)
- Government College University, Faisalabad (PK)
- King Saud University (SA)
- Municipality of Athens Development Agency (GR)
- University of Faisalabad (PK)
- Shenzhen Technology University (CN)
- University of Agriculture Faisalabad (PK)
- South China University of Technology (CN)
Publication Details
- Journal
- Cell Biochemistry and Biophysics
- Published
- 2025-01-27
- DOI
- https://doi.org/10.1007/s12013-024-01629-7
- Citations
- 1
- Primary Topic
- MicroRNA in disease regulation
- Type
- article
- Field-Weighted Citation Impact
- 0.94