Recombinase-based amplification coupled with lateral flow chromatography for the specific and sensitive detection and identification of Leishmania major in cutaneous leishmaniasis patients
This way, we retained one set for further investigation, which confirmed it is <i>L. major</i> species-specific. Tested on 86 human cutaneous samples, this selected set was able to detect 100% of <i>L. major</i> infections in confirmed CL patients. We did not observe any cross-reactivity with lesions due to <i>L. infantum</i> or <i>L. tropica</i>.
Authors
- Hejer Souguir (ORCID: https://orcid.org/0000-0002-4455-5900)
- Ahmed Sahbi Chakroun
- Insaf Bel Hadj Ali (ORCID: https://orcid.org/0000-0002-5289-5892)
- Emna Harigua‐Souiai (ORCID: https://orcid.org/0000-0003-2974-9157)
- Rhea N. Coler (ORCID: https://orcid.org/0000-0001-6974-5666)
- Aicha Kallel
- Steven G. Reed (ORCID: https://orcid.org/0000-0003-2193-100X)
- Hamed Chouaieb (ORCID: https://orcid.org/0000-0002-5920-6113)
- Meryem Lemrani (ORCID: https://orcid.org/0000-0002-9923-9219)
- Oussaïma El Dbouni
- Yusr Saadi Ben Aoun (ORCID: https://orcid.org/0000-0001-5850-4218)
- Nabil Haddad (ORCID: https://orcid.org/0000-0003-1430-9730)
- Ikram Guizani (ORCID: https://orcid.org/0000-0003-2763-8963)
- K. Kallel
- Akila Fathallah-Mili
- Imen Khammeri
Institutions
- Tunis University (TN)
- Infectious Disease Research Institute (US)
- Lebanese University (LB)
- University of Washington (US)
- Rafik Hariri University Hospital (LB)
- Institut Pasteur de Tunis (TN)
- Institut Pasteur du Maroc (MA)
- Tunis El Manar University (TN)
- University of Sousse (TN)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2025-01-27
- DOI
- https://doi.org/10.3389/fmicb.2024.1514684
- Citations
- 1
- Primary Topic
- Research on Leishmaniasis Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.88