The Effects of Microseconds Electroporation on Pore Size, Viability and Mitochondrial Membrane Potential of Cervical Cancer Cells

Objective: Electroporation (EP) is a method in which the membrane permeability is increased by applying electrical pulses.The determination of modifications that occur in cells subsequent to EP with varying pulse parameters holds significant importance in establishing the foundations of EP theory.Therefore, we sought clarification regarding the phenomenon of pore formation on the membrane of the electroporated human cervical cancer cell line (HeLa) cells. Methods:The pores created on the cell membrane due to EP was observed using a scanning electron microscope.The change in the viability and mitochondrial membrane potential (m) of cells was determined by WST-8 and JC-1 assays. Results:The surface of the electroporated cell membrane exhibited a relatively uniform pore population.The viability of HeLa cells was significantly reduced with increasing electric field intensities.A slight decrease in m was observed between the control and the 0.8 and 1.6 kV/cm EP groups, but m was higher in the 2.4 and 3.2 kV/cm EP groups compared to the control group. Conclusion:In conclusion, our study showed that the application of EP to the cervical cancer cell line resulted in the formation of pores of varying sizes on the membrane.While cell viability decreased with increasing electric field amplitude, no significant Z Ama: Elektroporasyon (EP), elektrik darbeleri uygulanarak membran geirgenliinin artrld bir yntemdir.EP sonras hcrelerde deien nabz parametreleriyle meydana gelen deiikliklerin belirlenmesi, EP teorisinin temellerinin oluturulmasnda byk nem tamaktadr.Bu nedenle, EP'ye tabi tutulan insan rahim az kanseri hcre dizisi (HeLa) hcrelerinin zar zerinde gzenek oluumu olgusuna ilikin aklama aradk.Yntemler: EP'ye bal olarak hcre zarnda oluan gzenekler taramal elektron mikroskobu kullanlarak gzlemlendi.Hcrelerin canll ve mitokondriyal membran potansiyelindeki (m) deiiklik, WST-8 ve JC-1 analizleri ile belirlendi.Bulgular: EP'ye tabi tutulmu hcre zarnn yzeyi nispeten tekdze bir gzenek poplasyonu sergiledi.HeLa hcrelerinin canll, artan elektrik alan younluklaryla nemli lde azald.Kontrol grubu ile 0,8 ve 1,6 kV/cm EP gruplar arasnda m'de hafif bir azalma gzlendi ancak 2,4 ve 3,2 kV/cm EP gruplarnda m kontrol grubuna gre daha yksekti.