WCN25-1344 GUT MICROBIOTA AND BLOOD METABOLIC PROFILE IN PATIENTS ON PERITONEAL DIALYSIS

For the negative control (NC), we prepared a buffer containing 1.06% dextrose by mixing 4.25% dextrose DELFLEX (Fresenius Medical Care, 054-20224) and 1xPBS (pH 7.2) in a 1:3 ratio.For the positive control (PC), WBCs were purified from the blood of a healthy donor, resuspended in the NC buffer, diluted to 100 cells/mL (PDRP threshold per ISPD guidelines), aliquoted, and stored at -80 C until analysis.For the leukocyte strip test, a thawed PC, an NC, and six thawed spent PDE (250 mL each) were arranged in a row.Eight urinary test strips containing leukocytes test pads (LotFancy, 11J-2136-D.1)were simultaneously dipped into samples for two seconds, then laid flat while a video was recorded to capture the color changes over 10 minutes.Signal intensities were analyzed by ImageJ (https://imagej.net/).