Review on the Conservation and in vitro Regeneration of Medicinal Plant Boerhavia diffusa

Boerhavia diffusa (Punarnava) is a flowering herbal plant belonging to Nyctaginaceae family. This species holds a significant medicinal importance across various cultural and traditional medicinal systems, including Ayurveda and Unani, where its aerial parts and roots are utilized to treat a wide range of ailments in humans and animals. One of the key reasons behind the plant popularity is its significant pharmacological importance because of the diverse array of biologically active compounds present in. its various parts such as roots, leaves, stem, and seeds. This very popularity makes it a sought-after resource for pharmaceutical industries and, therefore, has led to detrimental consequences, including over-exploitation and habitat degradation. In vitro culture technique provides a promising avenue for the conservation and sustainable production of this valuable herb. The tissue culture method is beneficial for the sustained production of phytochemicals as well as for its conservation. This review is based on the morphological and taxonomical status along with the medicinal importance, and the scientific aspects of tissue culture protocols developed for B. diffusa. . KEYWORDS :Punarnava, Nyctaginaceae, Medicinal plant, In vitro regeneration