Ribosome pausing in amylase producing Bacillus subtilis during long fermentation
Expression of the α-amylase gene amyM in B. subtilis is accompanied by several ribosome pausing events. Since these sites can neither be predicted based on codon specificity nor on secondary protein structures, we speculate that secondary mRNA structures are responsible for these translation pausing sites. The detailed information of ribosome pausing sites in amyM provide novel information that can be used in future codon optimization studies aimed at improving the production of this amylase by B. subtilis.
Authors
- Paul Igor Costea (ORCID: https://orcid.org/0000-0003-1645-3947)
- Frans van der Kloet (ORCID: https://orcid.org/0000-0002-8573-2651)
- Yaozu Han
- Christopher Sauer (ORCID: https://orcid.org/0000-0002-3127-1706)
- Mathis Appelbaum
- Max Fabian Felle
- Leendert W. Hamoen (ORCID: https://orcid.org/0000-0001-9251-1403)
- Biwen Wang (ORCID: https://orcid.org/0000-0003-4383-3902)
- Gaurav Dugar (ORCID: https://orcid.org/0000-0002-5678-3679)
- Alberto Agnolin
Institutions
- Amsterdam University of Applied Sciences (NL)
- University of Amsterdam (NL)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-01-27
- DOI
- https://doi.org/10.1186/s12934-025-02659-3
- Citations
- 2
- Primary Topic
- Enzyme Structure and Function
- Type
- article
- Field-Weighted Citation Impact
- 1.04
Funders
- China Scholarship Council