High-performance thin-layer chromatography (HPTLC) fingerprinting and analytical evaluation of Vajra Guggulu: a Herbo-mineral formulation

Introduction: Vajra Guggulu is a classical Ayurvedic formulation in which every medication comprises a combination of chemical components with proven neuroprotective properties. The study's goals were to perform HPTLC to identify phytochemicals in Vajra Guggulu and physiochemically analyze the formulation of Vajra Guggulu according to the Ayurvedic Pharmacopoeia of India (API) standards. Methods: Vajra Guggulu was prepared according to the reference provided in the Ayurvedic classical text Ras Ratnakar, and its quality was evaluated using organoleptic characteristics and physiochemical specifications as described in API, followed by Standard HP-TLC analysis on the samples. Results: The physicochemical evaluation of Vajra Guggulu confirms its authenticity and quality. HPTLC analysis reveals the presence of phytochemicals that can delay the progression of inflammatory, peripheral vascular, and neurological illnesses. Conclusion: This study will serve as a reference standard for medicine manufacturing, quality analysis, and assessing Vajra Guggulu's future therapeutic potential.