AN EXPERIMENTAL MODEL FOR STUDYING THE RESPONSE OF CELLS TO THE EFFECTS OF PERIODONTAL GEL COMPOSITIONS AND BRACES POTENTIATED BY ELECTROPHORESIS

Aim: Іnvestigation of the effectiveness of penetration of a dental gel composition based on a flavonoid complex and benzydamine hydrochloride (patented periodontal gel composition Benzidaflaziverdin (GCB)) into the simulated environment of periodontal tissues consisting of three types of mammalian and human cells in semi-solid agar using an electrophoresis procedure. Research methods. The penetration of GCB and comparison drugs (Сholisal, Gengigel®) into the microenvironment of periodontal tissues was explored in vitro, consisting of three types of cells grown in semi-solid agar. These are murine BALB-3T3 fibroblasts, murine J774.2 macrophages, and pseudo-normal human HaCaT keratinocytes. Electrophoresis was conducted using the Potik-1 device (SMEP). Unused and used orthodontic braces were applied in the oral cavity to assess the influence of metal elements in the cellular microenvironment. Cell viability was quantified using an MTT test. The reactive oxygen species (ROS) level was determined after cell staining with fluorescent dye dihydroethidium (DHE). Results: GCB showed a higher ability to promote the proliferation of all studied cells compared with Cholisal and Gengigel® drugs. Electrophoresis potentiated cytostimulatory and protective effects of GCB when applied to + electrode. This evidences that the duration of electrophoresis conducted in clinics can be reduced from 15–20 min per jaw to 15–50 sec. In comparison, the prolongation of GCB action and local delivery of flavonoid complex and benzydamine hydrochloride into the microenvironment were maintained. Unused braces were shown to lose metal cations more intensively in the culture microenvironment, thus increasing oxidative stress. It is suggested that GCB modulates the ability of cells to withstand oxidative stress. Conclusions: GCB may be recommended as a new product for periodontal dressing in clinical periodontics and orthodontics.