“SYMBION-D” INFLUENCE ON THE INTESTINE MICROBIAL COMPOSITION AND IMMUNITY OF CALVES WITH DYSPEPSIA

The purpose of research is to study the effect of a biologically active composition of microbial origin (BACMO) “Symbion-D” on the microbial composition of the intestine and the immunity of calves with dyspepsia. The studies were carried out on calves of the Holstein breed of the neonatal period, belonging to the Federal State Unitary Enterprise Omskoye. The criteria for inclusion in the experimental group of animals was the clinical sign "diarrhea". The calves of the experimental group were fed BACMO “Symbion-D” daily for 5–7 days individually, depending on the weight of the calf and the severity of the disease. To study the microbial composition of the intestines, fecal samples were taken from three animals, and the immune status was taken from five animals randomly from each experimental group. It was established that BACMO “Symbion-D” contributes to the reduction of microorganisms of the genus Staphylococcus epidermidis from 1×105 to 1×103 CFU/g and the genus Proteus spp. up to 103 CFU/g. The content of microorganisms of the genus Lactobacillus spp. increased from ×105 to ×108 CFU/g. When assessing the immune status of sick calves against the background of the use of BACMO “Symbion-D”, a significant increase in the number of lymphocytes by 15 % (p≤0.05) compared with the background values and T-lymphocytes by 52 % was recorded, which indicates the activation of immune cells, as well as a decrease in the number of cytotoxic T-lymphocytes in the blood by 20 % relative to background indicators. In addition, “Symbion-D” activated the functional activity of leukocytes, as evidenced by an increase in the level of lysosomal cationic proteins by 7 %. A significant trend towards a decrease in spontaneous NBT activity and an increase in the functional reserve of neutrophils indicates a decrease in the antigenic load on the body of calves.