EXPERIMENTAL VALIDATION OF THE USE OF POLARISATION TOMOGRAPHY OF POLYCRYSTALLINE COMPONENT OF BLOOD FILMS IN THE DIFFERENTIAL DIAGNOSTICS OF THE SEVERITY OF THE COURSE OF ABDOMINAL SEPSIS

Introduction. Assessment of the severity of enteric insufficiency in abdominal sepsis requires improvement, as a differentiated approach to the treatment of such patients depends on it. Therefore, the use of polarization tomography of the polycrystalline component of blood films is one of the promising directions for diagnosing the severity of the course of abdominal sepsis. Aim. To investigate in an experiment the method of polarization tomography of the polycrystalline component of blood films as a diagnostic criterion for determining the severity of the course of abdominal sepsis. Materials and methods. We modelled mild, moderate and severe peritonitis on 80 white non-linear rats of both sexes, weighing from 180 to 220 g, according to the methodology developed in the clinic. Samples of blood films from rats with different severity of peritonitis were used as objects of research using polarization tomography of the polycrystalline component of blood films and determination of distributions of phase parameters in pixels of a digital image. Results. The conducted experimental studies showed that the system of polarization mapping of the polycrystalline component of the blood films of laboratory rats clearly determines the degree of intoxication depending on the severity of abdominal sepsis, which is visualized by obtaining an album of maps of distributions of phase values of digital microscopic image points. The most diagnostically sensitive statistical criteria for differentiation of phase maps of microscopic images of blood films of rats from control group 1 and experimental groups 2-4 were found, which contributed to the determination of relationships between the distributions of statistical parameters of the 1st-4th orders, which characterize the phase maps depending on the severity of peritonitis. Conclusions. The transformation of the statistical structure of linear birefringence polarization tomograms of the blood of rats is associated with necrotic degradation (anemia) of optically anisotropic hemoglobin structures, as well as with a decrease in the number of optically active erythrocytes in the blood of rats changed as a result of abdominal sepsis, depending on the severity of its course. The balanced accuracy of differentiation of rats of the control and experimental groups, the intergroup differentiation of the course of abdominal sepsis depending on the degree of severity testify to the presence of the diagnostic ability of the polarization tomography method.