Application of CRISPR/Cas-based Biosensing Platforms in Molecular Diagnostics

The CRISPR/Cas system is widely used in genome editing. The CRISPR/Cas system can precisely cleave and recognize DNA and RNA sequences. In the process of recognizing a target sequence, the CRISPR/Cas system not only recognizes the target sequence and initiates target-cleaving activity, but also shows non-specific cleavage activity on single strands such as DNA and RNA molecules, and emits fluorescence. is appended. Group of reporter nucleic acid molecules to be added in the reaction system, the positions of target nucleic acid molecules can be observed by fluorescence signals, and multiple target molecules can be detected simultaneously due to the different cleavage priorities of Cas proteins.