OPTIMIZATION OF SOME FACTORS IN ORDER TO REGENERATE USING EMBRYO CULTURE IN SOME PLUM AND CHERRY GENOTYPES

In last year’s, it has been observed that the germination rate of the resulting stones in hybrid combinations is very low. Starting from this problem, was checked a different solution than classic to obtain hybrid plants, solution that offers and analysis that gives as many explanations for this situation. The biological material used was represented by 1,140 plum hybrids stones (resulted from different hybrid combinations - ‘Andreea x Romanța’, ‘Pitestean x Andreea’, ‘Tita x Jojo’, and open pollination of ‘Stanley’, ‘Jojo’, ‘Anna Spath’, ‘Tamaioasă de Bistrita’, ‘Mildora’ cvs.) and 815 sweet cherry hybrids stones resulted from open pollination of ‘Skeena’, ‘Superb’, ‘Stella’, ‘Kordia’, ‘Severin’ cvs. The method used for regeneration was embryo culture with 2 variants of culture medium based on Lee Fossard components and hormonal balances, which included IBA, BAP and GA3. In plum, 40.35% of the stones were unsuitable for inoculation due to dehydration, which also led to a very small percentage of regenerated plants of only 3.97%, and in sweet cherry, was recorded 61.96 % dehydrated stones and the germination percent was 13.23%.