Ultrathin 2D Cu-Porphyrin MOF Nanosheet Loaded Fe₃O₄ Nanoparticles As a Multifunctional Nanoplatform for Synergetic Chemodynamic and Photodynamic Therapy Independent of O₂

In this study, we developed a multifunctional nanoplatform to address the limitations of strictly acidic pH for the Fenton reaction involving Fe₃O₄ and the low efficiency of mono treatments. The hybrid material, Fe₃O₄@Cu-TCPP, was assembled through hydrophobic interactions of polyvinylpyrrolidone (PVP) coated on its surface. The efficiency of the Fenton reaction using Fe₃O₄ was significantly enhanced by the photo-Fenton process in the presence of Cu-TCPP. The generation of hydroxyl radicals by Fe₃O₄@Cu-TCPP was markedly increased under laser irradiation (λ = 660 nm) in solution. Fe₃O₄@Cu-TCPP demonstrated a robust ability to produce reactive oxygen species through chemodynamic and photodynamic processes independent of that of O₂. <i>In vivo</i> experimental results indicated that Fe₃O₄@Cu-TCPP facilitated <i>T</i>₂-weighted magnetic resonance imaging-mediated synergistic chemodynamic and photodynamic therapies in a 4T1 mouse model.