Enhanced fluorescence detection of tau protein using photonic crystal-based biochips: a novel tool for Alzheimer's disease diagnostics
In this study, we present an innovative approach for the detection of Tau protein, a key biomarker of Alzheimer disease, using enhanced fluorescence detection. The methodology utilizes a bio-conjugation process to adapt 1D photonic crystal surface for precise molecular recognition. Our results demonstrate the successful detection of Tau protein in a simple environment, with a detection limit of 0.5ng/mL and a quantification limit of 1.2ng/mL. This work introduces optical disposable biochips as a novel diagnostic tool for Alzheimer disease and related neurodegenerative disorders, addressing critical challenges in biomarker detection and paving the way for advancements in therapeutic development.
Authors
- Alberto Sinibaldi (ORCID: https://orcid.org/0000-0003-1970-3737)
- Francesco Baldini (ORCID: https://orcid.org/0000-0002-2689-5952)
- Agostino Occhicone (ORCID: https://orcid.org/0000-0001-8295-6222)
- Francesco Chiavaioli (ORCID: https://orcid.org/0000-0002-3169-7395)
- Ambra Giannetti (ORCID: https://orcid.org/0000-0002-0002-2977)
- Francesco Michelotti (ORCID: https://orcid.org/0000-0003-0285-6059)
- Peter Munzert
- Norbert Danz (ORCID: https://orcid.org/0000-0002-8927-9284)
Institutions
- Nello Carrara Institute of Applied Physics (IT)
- Fraunhofer Institute for Applied Optics and Precision Engineering (DE)
- Center for Nano Science and Technology (IT)
- Sapienza University of Rome (IT)
Publication Details
- Published
- 2025-01-23
- DOI
- https://doi.org/10.1117/12.3058708
- Citations
- 1
- Primary Topic
- Advanced Biosensing Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.81