Understanding therapeutic nuclear magnetic resonance (tNMR): splitting of components indicates its unique efficacy

Therapeutic Nuclear Magnetic Resonance (tNMR, MBST®, Wetzlar, Germany) was derived from MRI, reducing the magnetic field intensity to 0.4mT and the radiofrequency to <18kHz, to induce water proton resonance conditions. However, it was unknown whether proton resonances were essential for the manifold cellular effects observed, such as changes in the circadian clock, the hypoxic signaling pathway and in reactive oxygen species (ROS). Therefore, chip cards were designed to selectively induce either tNMR, the sweep field (0.4mT, 50Hz) or the radiofrequency (up to 18kHz). Preliminary data now indicate distinct cellular responses for each of the three modes, regarding ROS partitioning, lactate metabolism, and cellular proliferation rates. Interestingly, tNMR affected cellular processes known to require proton gradients, such as mitochondrial respiration and lactate shuttling via proton-coupled Monocarboxylate Transporters. This suggests that tNMR potentially enhances cellular proton motive forces. Conclusively, our results highlight a huge potential for all three applications, tNMR, sweep field and radiofrequency, and emphasize the unique role of tNMR.