Characterization of Pure Ozonides from Ozonolysis of Oleic Acid Methyl Ester; Use of a Protocol for the Analysis of the Corresponding Stable Ozonides from Triolein and Organic Extra Virgin Olive Oil (+OIL®)

As part of the research directed at establishing the composition of the main products present in Ozoile^®, a study of the ozonolysis of the oleic acid methyl ester as well as triolein was undertaken. Starting from oleic acid methyl ester, all six ozonides were isolated for the first time and fully characterized. Then, we used a protocol based on ozonolysis of triolein and +OIL followed by trans-esterification of the crude reaction mixtures, which led to the same six ozonides. Furthermore, to exclude the formation of any other oxygenated compounds, both during the ozonolysis process and afterward, the reactivity towards ³O₂ and ¹O₂ was explored. The ozonolysis of oleic acid methyl ester in a participating solvent (MeOH) was also investigated.