Exploring Volatiles and Biological Effects of Commiphora africana and Boswellia papyrifera Incense

The resin of <i>Commiphora africana</i> and the resin and bark of <i>Boswellia papyrifera</i> play versatile roles in traditional Sudanese culture, including use in inhalation therapy, liquid remedies, and as chewing gum. Thus, this study aimed to analyze the volatile compounds in these materials using various extraction methods and assess their biological activities. Extraction methods included MonoTrap solid-phase microextraction, smoke solvent trapping, and acetone immersion. Gas chromatography-mass spectrometry analysis of MonoTrap extracts identified highly volatile compounds, while smoke extracts contained compounds with lower volatility. Solvent immersion captured a broader range of compounds. The resin of <i>C. africana</i> was rich in limonene, verbenone, and β-selinene, whereas <i>B. papyrifera</i> extracts contained octyl acetate, trans-nerolidol, and nerolidol isobutyrate as major compounds. Biological assays showed <i>C. africana</i> smoke extract inhibited tyrosinase activity, with <i>p</i>-cymene and <i>S</i>-limonene acting as competitive inhibitors. It also inhibited the growth of cancer cells, A549 and MIA Paca-2, while solvent extracts from both resins inhibited all tested cell lines. Further, the acetone extracts exhibited strong antibacterial activity against <i>Streptococcus mutans</i>. These results highlight the differences in chemical composition between the two species, the impact of extraction methods, and the therapeutic potential of <i>C. africana</i> and <i>B. papyrifera</i> as sources of bioactive compounds.