Streptococcus suivaginalis sp. nov., Streptococcus iners sp. nov. and Streptococcus iners subsp. hyiners subsp. nov. isolated from pigs

Three novel strains within the genus <i>Streptococcus</i> (29887^T, 29892^T and 29896^T) were isolated from healthy pigs during routine veterinary physical exams. All three strains were non-motile and non-spore-forming Gram-positive cocci. The complete genome of each strain was attained, and phylogenetic analyses were performed. Comparison of the genomes of 29887^T, 29892^T and 29896^T to the genomes of other <i>Streptococcus</i> strains revealed digital DNA-DNA hybridization (dDDH) values between 21.2% and 53.9% and average nucleotide identity (ANI) values between 70.00% and 94.44%. Phylogenetic analyses suggested that each strain, 29896^T (<i>S. suivaginalis</i> sp. nov.), 29887^T (<i>S. iners</i> sp. nov.) and 29892^T (<i>S. iners</i> subsp. <i>hyiners</i> subsp. nov.), may represent a novel species within the genus <i>Streptococcus</i>, while ANI analysis indicated that strains 29896^T (<i>S. suivaginalis</i> sp. nov.) and 29887^T (<i>S. iners</i> sp. nov.) represent novel species within the genus <i>Streptococcus,</i> and 29892^T (<i>S. iners</i> subsp. <i>hyiners</i> subsp. nov.) represents a novel subspecies of 29887^T (<i>S. iners</i> sp. nov.). Based upon the combined data presented in this study, two novel species, <i>Streptococcus suivaginalis</i> sp. nov. (type strain, 29896^T=NRRL B-65677^T=NCTC 14941^T) and <i>Streptococcus iners</i> sp. nov. (type strain, 29887^T=NRRL B-65675^T=NCTC 14939^T) are proposed, and one novel subspecies, <i>Streptococcus iners</i> subsp. <i>hyiners</i> subsp. nov. (type strain, 29892^T=NRRL B-65676^T=NCTC 14940^T) is proposed.