Elucidating assembly and function of VirB8 cell wall subunits refines the DNA translocation model in Gram-positive T4SSs
Bacterial type IV secretion systems (T4SSs) are widespread nanomachines specialized in the transport across the cell envelope of various types of molecules including mobile genetic elements during conjugation. Despite their prevalence in Gram-positive bacteria, including relevant pathogens, their assembly and functioning remain unknown. This study addresses these gaps by investigating VirB8 proteins, known to be central components of conjugative T4SSs in Gram-positive bacteria. However, the functional packing and precise role of VirB8 in T4SSs biology remain undefined. Our findings elucidate the nature of VirB8 proteins as cell wall components, where they multimerize and exhibit a conserved assembly pattern, distinct from VirB8 in Gram-negative bacteria. We also demonstrate that VirB8 proteins interact with other T4SS subunits and DNA, indicating their pivotal role in the building of the DNA translocation channel across the cell wall. We lastly propose a distinct architecture for conjugative T4SSs in Gram-positive bacteria compared to their Gram-negative counterparts, possibly attributed to the differences in the cell wall structure.
Authors
- Rémi Fronzes (ORCID: https://orcid.org/0000-0003-3031-9824)
- Haifa Laroussi
- Sandrine Mathiot
- Johannes Hüebner (ORCID: https://orcid.org/0000-0003-1232-4646)
- Nathalie Leblond‐Bourget (ORCID: https://orcid.org/0000-0001-5739-1370)
- Abbas M. Ali (ORCID: https://orcid.org/0000-0003-3037-4903)
- Julien Cappèle
- Claude Didierjean (ORCID: https://orcid.org/0000-0002-3176-8974)
- Emilie Robert (ORCID: https://orcid.org/0009-0002-2848-111X)
- F. Favier (ORCID: https://orcid.org/0000-0003-3249-997X)
- Badreddine Douzi (ORCID: https://orcid.org/0000-0003-3140-4713)
- Yvonne Roussel (ORCID: https://orcid.org/0000-0003-3191-043X)
- Nancy E. Ramia
- Felipe Romero‐Saavedra (ORCID: https://orcid.org/0000-0003-3134-7018)
- Nicolás Soler (ORCID: https://orcid.org/0000-0002-4292-4020)
- Robine Maffo-Woulefack (ORCID: https://orcid.org/0000-0003-2746-0229)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université de Bordeaux (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Laboratoire de Cristallographie, Résonance Magnétique et Modélisations (FR)
- Dynamique des Génomes et Adaptation Microbienne (FR)
- Institut Européen de Chimie et Biologie (FR)
- Institut Polytechnique de Bordeaux (FR)
- Laboratoire de Microbiologie Fondamentale et Pathogénicité (FR)
- Ludwig-Maximilians-Universität München (DE)
- Université de Lorraine (FR)
Publication Details
- Journal
- Science Advances
- Published
- 2025-01-22
- DOI
- https://doi.org/10.1126/sciadv.adq5975
- Citations
- 5
- Primary Topic
- Bacterial Genetics and Biotechnology
- Type
- article
- Field-Weighted Citation Impact
- 9.09