Selection and evaluation of black short grain breeding lines associated with yield, cooking quality, high nutrition and antioxidant potential derived from <i>indica</i> black rice x <i>japonica</i> white rice

Abstract The combination of the trend of Japanese food consumption with the health benefits of black rice is in high demand for rice consumers in Thailand. For this challenge, incorporation of desirable traits from tropical indica black rice, Riceberry and temperate japonica white rice, Akitakomachi was performed by pedigree selection with maker-assisted selection (MAS). The three candidate lines showed highly favourable agronomic characteristics and a high grain yield, with short grains and good cooking quality, similar to japonica rice, in a tropical climate. In addition, these lines showed black coloration of the pericarp, indicating high nutritional value and phytochemical, antioxidant and antidiabetic activities, similar to those of the Riceberry parent. In terms of the sensory testing of unpolished rice, two breeding lines; 69-1-1 and 72-4-3 showed higher scores than their parents. However, only 69-1-1 was identified as japonica type according to its genetic background. Therefore, this breeding programme can create black short grain rice variety adapted to a tropical environment, similar to japonica-type rice.