Single-Cell Proteomics Reveals Specific Cellular Subtypes in Cardiomyocytes Derived From Human iPSCs and Adult Hearts
Single-cell proteomics was performed on human induced pluripotent stem cells (iPSCs), iPSC-derived cardiomyocytes, and adult cardiomyocytes. More than 700 proteins could be simultaneously measured in each cell revealing unique subpopulations. A subset of iPSCs expressed higher levels of Lin28a and Tra-1-60 towards the outer edge of cell colonies. In the cardiomyocytes, two distinct populations were found that exhibited complementary metabolic profiles. Cardiomyocytes from iPSCs showed a glycolysis profile while adult cardiomyocytes were enriched in proteins involved with fatty acid metabolism. Interestingly, rare single cells also co-expressed markers of both cardiac and neuronal lineages, suggesting there may be a novel hybrid cell type in the human heart.
Authors
- Lizhuo Ai (ORCID: https://orcid.org/0000-0001-5449-9888)
- Jennifer E. Van Eyk (ORCID: https://orcid.org/0000-0001-9050-148X)
- Aleksandra Binek (ORCID: https://orcid.org/0000-0001-5764-7137)
- Niveda Sundararaman (ORCID: https://orcid.org/0000-0001-7708-3754)
- Madelyn Arzt
- Simion Kreimer (ORCID: https://orcid.org/0000-0001-6627-3771)
- Ali Haghani
- Blandine Chazarin
- Eduardo Marbán (ORCID: https://orcid.org/0000-0002-3863-280X)
- Arun Sharma (ORCID: https://orcid.org/0000-0002-0607-5455)
- Vladimir Zhemkov (ORCID: https://orcid.org/0000-0002-6554-1938)
- Clive N. Svendsen (ORCID: https://orcid.org/0000-0001-8696-3446)
- Edo Israely
- Jae Hyung Cho (ORCID: https://orcid.org/0000-0002-3851-2947)
Institutions
- Cedars-Sinai Medical Center (US)
- Cedars-Sinai Smidt Heart Institute (US)
Publication Details
- Journal
- Molecular & Cellular Proteomics
- Published
- 2025-01-22
- DOI
- https://doi.org/10.1016/j.mcpro.2025.100910
- Citations
- 11
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 8.87
Funders
- Foundation for the National Institutes of Health
- National Aeronautics and Space Administration
- California Institute for Regenerative Medicine
- American Heart Association
- Cedars-Sinai Medical Center
- National Institutes of Health