Effects of exosomes derived from MSC-iPSC on fibroblasts in vitro in the presence of platelet-rich plasma

Mesenchymal stem cells, as well as mesenchymal stem cells-induced pluripotent stem cells, can definitely induce alterations of fibroblasts and osteoblasts micro medium, stimulating the development of bone around the dental implants.Aim: The purpose of the current study was represented by the following of the effects of stimulation and inhibition of multiple intracellular fun ctional pathways of senescent gingival fibroblasts in culture when exosomes released from plurip otent stem cells reprogrammed from mesenchymal stem cells and platelet-rich plasma were used.Materials and methods: We used flow cytometry to measure the production of the beta-galactosidase level by gingival fibroblasts in culture after 30 passages and ultravi olet treatment.Results: Reduction of -galactosidase concentrations in senescent gingival fibroblasts was evident upon administration of platelet-rich plasma, exosomes and exosomes coadministered the same time with: LY-294002, nutlin-3, berberine, resveratrol, collagen, thrombin, and fibronectin.On the other side, lipopolysaccharide (which might induce a pr oinflammatory profile on iPSC-MSC) enhanced the production of -galactosidase.The most intense effects were associated with collagen, thrombin, fibronectin, resveratrol , and berberine.Conclusions: Exosomes isolated from pluripotent stem cells derived from mesenchymal stem cells, administered in the culture medium of senescent gingival fibroblasts, can modify the degree and evolution of the senescence of these last cells by potentiating, activating, or stimulating some intracellular pathways of biological signal transduction.