Determination of Biologically Active Substances in the Fruits of Sea Buckthorn (Hippophaes Rhamnoides L.) growing in the Lori and Sevan regions

Key words: medicinal plant, flavonoids, quantitative determination, spectrophotometry, tannins, carotenoids The content of biologically active substances (carotenoids, ascorbic acid, calcium) in the fruits of sea buckthorn (Hippophaes Rhamnoides L.), growing in the Lori and Sevan regions, had been studied using titrimetric and spectrophotometric meth,ods. Their quantitative content in the fruits of the medicinal plant was determined and a comparative analysis was carried out. The results of determining the calcium content in the studied samples show that its amount in sea buckthorn fruits, collected from two different places with significantly different climatic conditions, is comparable, while it practically does not change depending on the drying process. The results of determining the content of ascorbic acid show that its amount in sea buckthorn fruits collected from two different places with significantly different climatic conditions varies significantly: it is high in fruits collected from the Sevan region, and drying processes have virtually no effect on this. The spectrophorometric method was used for quantitative determination of carotenoids. Optical density was measured on a SPECORD-UV-VIZ spectrophotometer at a wavelength of λ = 450 nm. Studies have shown that sea buckthorn fruits collected from the Sevan region have a high carotenoid content. At the same time, it was also found that the drying process leads to a decrease in the amount of carotenoids in the samples.