Pharmacokinetic parameters of caffeine in cattle in the context of liver functional assessment

A retrospective analysis of the scientific literature indicates that the predictive potential of caffeine in assessing the safety of the metabolic function of the hepatobiliary system has been actively studied in some animal species (laboratory animals, horses, etc.), but such studies were few in number and ambiguous in terms of obtaining a stable diagnostic result, which allows us to consider further experiments of this kind promising. The main objective of this study is to identify standard pharmacokinetic parameters of caffeine in clinically healthy animals (cattle) for their further use as a starting point for further study of changes in these parameters in various pathologies of the hepatobiliary system. The studies were conducted in 2024 in one of the livestock farms of the Pskov region of the dairy industry; Holsteinized cattle were used in the experiments. In experimental animals pre-treated with exogenous caffeine, after 1, 2, 3, 4, 5, 6, 8, 10, 12, 18 and 24 hours, blood was collected from the experimental animals by venipuncture of the jugular vein, after which caffeine levels were determined by enzyme immunoassay. The obtained data were statistically processed using open software for calculating pharmacokinetic parameters ("distribution volume", "total plasma clearance" and "mean residence time"). The obtained pharmacokinetic parameters, as well as the caffeine levels themselves, will be the starting point for further studies, since in order to identify patterns of change in the behavior of the dynamic clearance test with caffeine, it is necessary to take into account the change in these indicators in a comparative aspect with animals with susceptible hepatopathies.