Histological and immunohistochemical changes in gonadotropic endocrinocytes of the pituitary gland of female rats exposed to dark deprivation

The effect of prolonged light desynchronosis on the body requires further study to understand its effect on the reproductive system and to study the adaptation of the body against this background, after the abolition of dark deprivation. The aim is to identify histological and immunohistochemical changes in gonadotropic endocrinocytes of the adenohypophysis of female rats under the influence of dark deprivation. The study involved 18 sexually mature white female rats (control group, n = 6) was under artificial lighting 12/12 (light/dark) for 30 days, experimental groups 1 and 2 – under 24-hour artificial lighting 24/0 (light/dark), then the animals of group 2 were placed in 12/12 mode for 14 days. 30-day dark deprivation caused an increase in FSH levels, an increase in the area, volume and perimeter of gonadotropic endocrinocytes by 19.95 % (p 0.001), 73.4 % (p 0.001), 18.2 % (p 0.001), respectively; an increase in the area, volume and perimeter of the core by 26.82 % (p 0.05), 26.82 % (p 0.001), 10.07 % (p 0.05), respectively. Immunohistochemical examination showed an increase in the specific area of caspase-3 of immunoreactive gonadotropic endocrinocytes by 22.4 % (p 0.05), a decrease in the expression of Klotho protein by 21.2 % (p 0.05). 14 days after the cancellation of dark deprivation, the restoration of FSH levels and the size of gonadotropic endocrinocytes was revealed.