Electrochemical Resolution of Ascorbic Acid from Dopamine and Uric Acid with Montmorillonite k10-Clay/Poly (Glycine) Electrode

Ascorbic acid is a physiologically important biomolecule, acting as an antioxidant and controlling conditions like scurvy, cancer and AIDS. Hence, it is crucial to estimate its levels in body fluids to detect any disorders. In this study, a cost-effective cyclic voltametric method was used to determine ascorbic acid in the presence of uric acid and dopamine. A new modified carbon paste electrode, namely Montmorillonite K10-clay/Poly, was fabricated for this purpose. The obtained modified electrode exhibits excellent electrocatalytic activity for the selective detection of ascorbic acid, even in the presence of uric acid and dopamine. Its sensitivity for ascorbic acid is doubled compared to the bare carbon paste electrode. The detection limit and quantification limit of this electrode for ascorbic acid are found to be 1.13×10-6 M and 3.77×10-5 M respectively. Additionally, the modified electrode recovers 96.5% of ascorbic acid in real sample analysis, such as the analysis of a vitamin C tablet (Celin-500mg). The selectivity, reproducibility and sensitivity of this modified electrode provide a promising approach for applying the technique in routine analysis of selected electroactive biomolecules in clinical and bioanalytical laboratories.