Comparative assessment of immune profile in children under the influence of benz(a)pyrene and cold factor in a case-control study with modeling and verification of the results obtained in an in vivo experiment

The comparative assessment of immune profile indicators in children under = benzo(a)pyrene and cold factor influence in a case-control study with the modeling and verification of obtained results in an in vivo experiment was performed. In the course of a case-control study in children under airborne benzo(a)pyrene exposure the signs of phagocytic leukocyte activity inhibition and specific haptenic hypersensitivity formation (IgG to benzo(a)pyrene) associated with blood contamination with benzo(a)pyrene were established. Revealed changes in the immune profile and xenobial biomedia composition in children under airborne benzo(a)pyrene exposure at a dose of 0.00761 mcg/(kg*day) in the subarctic urbanized area, it is comparable to its level in children living under the conditions of external environmental benzo(a)pyrene exposure at a dose of 0.08689 mcg/(kg*day) in an urbanized area in a temperate climate zone (p>0.05). The results of the experimental study of the immunotropic effects of oral benzo(a)pyrene exposure at an average daily dose of 0.175 mcg/kg*day and cold factor influence (15.1±2.6 °C) in vivo also show the signs of phagocytosis inhibition in mice (OR=7.33 - 16.20, p<0.05). At the same time, the minimum values of phagocytosis percentage, phagocytic number and phagocytic index are observed with the combined benzo(a)pyrene and cold factor effect which confirms the hypothesis of immunotoxic benzo(a)pyrene exposure effect modification by a cold factor Keywords: benzo(a)pyrene; cold stress; children; immune profile; phagocytosis; in vivo experiment