Synthesis and Characterization of Selenium Nanoparticles Using Orange Peel Powder and Testing Its Inhibitory Activity on Some Bacterial Isolates

The present study aimed to synthesize selenium oxide nanoparticles (SeONPs) using aqueous extract orange peels extract which was used as reducing and covering agent. the characterization of particles done by series of tests. Examination p spectrometry Uv –Vis Where the highest peak of the ultraviolet spectrum appeared at the level of (241) with an absorbance of (3,93). infrared spectrometer examination (FT-IR) was among the clearly indicate that h a substance (Ascorbic and compounds of phenolic existing) aggregates hydroxyl in touch concluded that orange peels are responsible for the synthesis of nanoparticles and installed. X-ray diffraction measurement XRD crystal structure was spherical in shape, and the highest diffraction peak in the crystal plane was at the peak (72.7858) versus the diffraction angle. (32.855˚= 2θ) The range of values of crystalline levels (13.835˚- 103.019˚), which proves that all particles are within the Nanoscale. (FE-SEM) result showed that the nanoparticles sizes homogeneous and show that a crystal ball diameter ranges between (8.735 - 163 5nm) .After preparing a solution of selenium nanoparticles, its antibacterial activity was tested by measuring the inhibitory region of bacterial isolates from food sources towards each of the Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa) as well as Gram-positive bacteria (Staphylococcus aureus) , the results showed that the biosynthesized SeONPs showed an effective antibacterial activity against all tested bacteria respectively : Staphylococcus aureus , Pseudomonas aeruginosa , and Escherichia coli.