Estimation of Ursolic acid and Diosgenin in Herbal hair oil formulations used for Hair loss and Grey hair activity by using RP-HPLC Method

The essential oils have been used for many purposes due to their diverse pharmacological properties and morphological properties. Since it aids in the scalp's efficient absorption of nutrients and stimulates dormant hair follicles, the active fraction has been isolated in this study to examine its potential as a hair growth promoter. Additionally, the phytoconstituent or phytoconstituents responsible for hair growth activity have been identified. The current study's objective is to determine the amount of ursolic acid and dienin in several herbal hair oils with varying formulations using the HPLC technique. The various herbs and oils utilised in this study were used to make herbal hair oil that included important chemical components such as diosgenin and ursolic acid. These elements are in charge of promoting hair development and minimising grey hair. Studies on morphology, chemistry, and pharmacology assessed the oil. By using HPLC method the prepared oils are estimated for Ursolic acid and Diosgenin and one of the formulations has shown with good result.